1. Which technique separates charged particles using electric field?
- 1) Protein denaturing
- 2) Protein synthesis
- 3) Electrophoresis
- 4) Hydrolysis
Answer: 3)
Explanation: Electrophoresis is the technique that separates charged particles using electric field.
2. Electrophoresis was developed by:
- 1) Sanger
- 2) Tiselius
- 3) Tsvedberg
- 4) Tswett
Answer: 2)
Explanation: Tiselius developed the electrophoresis technique.
3. The speed of migration of ions in electric field depends upon:
- 1) Magnitude of charge and mass of molecule
- 2) Magnitude of charge shape and mass of molecule
- 3) Magnitude of charge and shape of molecule
- 4) Shape and size of molecule
Answer: 3)
Explanation: The speed will only depend on charge and shape. More the charge more will be the speed.
4. Which of the following statements is true about migration of biomolecules?
- 1) Rate of migration is inversely proportional to current
- 2) Low voltage is used for separation of high mass molecules
- 3) Rate of migration is directly proportional to current
- 4) The rate of migration is directly proportional to the resistance of medium
Answer: 3)
Explanation: More the current passing more will be the migration. Hence there is a direct proportion.
5. What does the electrophoresis apparatus consist of?
- 1) Power pack and electrophoresis unit
- 2) Electrophoresis unit and gel separator
- 3) Buffer chamber and electrophoresis unit
- 4) Gel, buffer chamber and fire pack
Answer: 1)
Explanation: The apparatus consists of power pack and electrophoresis unit.
6. If proteins are separated according to their electrophoretic mobility then the type of electrophoresis is:
- 1) Free flow electrophoresis
- 2) Electro focusing
- 3) Affinity Electrophoresis
- 4) SDS PAGE
Answer: 4)
Explanation: In the technique of SDS page, the proteins are separated according to their electrophoretic mobility.
7. The electrophoretic mobility denoted as µ is mathematically expressed as:
- 1) V/E
- 2) 1/EV
- 3) E/V
- 4) VE
Answer: 1)
Explanation: V/E is the formula for electrophoretic mobility which is the ratio of velocity of the biomolecule in the electric field and it's intensity.
8. Which of the following factors does not influence electrophoretic mobility?
- 1) Stereochemistry of molecule
- 2) Shape of molecule
- 3) Size of molecule
- 4) Molecular weight
Answer: 1)
Explanation: The stereochemistry of molecule won't have any effect on electrophoretic mobility since it is dependent on velocity and intensity and not alignment.
9. When is electrophoresis not used?
- 1) Separation of nucleic acids
- 2) Separation of Lipids
- 3) Separation of amino acids
- 4) Separation of proteins
Answer: 2)
Explanation: Electrophoresis cannot be used in separation of lipids.
10. What cannot be a reason for using electrophoresis?
- 1) Organizing DNA in order we can see
- 2) Organizing DNA fragments from largest to smallest
- 3) Organizing DNA by shape of backbone
- 4) Comparing two sets of DNA
Answer: 3)
Explanation: Electrophoresis cannot arrange molecules on shape of backbone.
